B.S. Abdur Rahman University,
Seethakathi Estate,
Vandalur,
Chennai -600048.
Phone :+91-44-22751347,48,50,75

Infrastructure&Equipments

State of Research laboratory facilities are available and equipped with sophisticated instruments

 

Generic Name of Equipment

Model, Make & year of purchase

CO2 Incubator

Model 3111, (ThermoFisher Scientific, USA), 2013

Phase Contrast Inverted Microscope

Axio Vert A1 FL (Carl Zeiss, Germany), 2013

Western Blot Semi Dry

TE70X (Hoefer, USA), 2013

Gel Electrophoresis Horizontal

SE250-10A-75 mighty small II mini vertical electrophoresis (Hoefer, USA), 2013

Gel Electrophoresis Vertical

SUB15 standard submarine gel system (Hoefer, USA), 2013

Gel Documentation Unit

GelDoc –IT imaging system (UVP LTD, UK), 2013

Phase Contrast Inverted Fluorescence Microscope

Axio Vert A1 FL (Carl Zeiss, Germany), 2013

Western Blot Semi Dry

Trans blot turbo (Biorad, USA), 2013

Western Blot wet

Standard transfer unit with cooling chamber (Hoefer, USA), 2013

Chemiluminiscent Gel Documentation Unit

ChemiDoc MP system with red module (Biorad,USA), 2013

Freeze Dryer

Alpha 2-4 Ldplus (Martin Christ, Germany), 2013

Real Time PCR

CFX96 (Biorad, USA), 2013

CO2 Incubator

Galaxy incubator 170R (Eppendorf, USA), 2013

Deep Freezer -80C

Premium upright freezer U410 (Eppendorf, USA), 2013

Deep Freezer -20C

Revco -20 (ThermoFisher Scientific, USA), 2013

High performance liquid chromatography (HPLC)  With PDA & Fluorescence Detector

LC-2010CHT (Shimadzu, Japan), 2013

Gel Electrophoresis Horizontal

Mini Sub GT Cell (Biorad, USA), 2013

Gel Electrophoresis Vertical

Mini-PROTEAN Tetra (Biorad, USA), 2013

Manual Colony Counter

Scan 100 (Intersciences, France), 2013

Multiporator

Multiporator, for eukaryotics, bacteria and yeasts (Eppendorf,USA), 2013

Spectrophotometer

BioSpectrometer basic  (Eppendorf, USA), 2013

Multimode Reader With Alpha Tech

Enspire multilabel plate reader with Alpha Tech (PerkinElmer, USA), 2013

PCR

Mastercycler nexus gradient (Eppendorf, USA), 2013

 

Infrastructure and Labs

The school has sufficient number of well equipped class rooms, fully equipped laboratories with state of the art equipments, conference hall, school and library. The school has the following laboratories adequately equipped with latest equipments

  • Molecular biology lab
  • Biochemistry lab
  • Genetics lab
  • Microbiology lab
  • Computational biology lab
  • Plant tissue culture lab
  • Animal tissue culture lab
  • Central research laboratory

 

Equipments

 

Chemi-luminescent Gel-documentation Unit, BioRad, USA

  • Gel imaging system, Automated, hands-off routines

  • Save and recall all the steps in the workflow for repeatable and reproducible results.

  • Comprehensive, automated quantitative analysis of protein and DNA samples

 


Phase Contrast Inverted Fluorescence Microscope, Carl Zeiss, Germany

  • The phase contrast microscope was developed visualize specimens that fluoresce, that is, emit light of a different color than the light absorbed by the specimen. 

  • This microscope has stable time-lapse imaging with automatic focus correction system.

 


Lyophilizer, Martin Christ

  • Lyophilized (freeze dried) products retain their original properties. 
  • There is no need for cooling during storage
  • There is a wide range of substances for which lyophilization is used as conservation / preservation method
  • In the pharmaceutical and biotechnology industries it is widely used for the following products: antibiotics, bacteria, vaccines, hormones, vitamins, enzymes, ferments, peptides, liposomes, collagen, plant extracts, blood plasma and antibodies.

 


Mastercycler nexus gradient, Eppendorf India Limited

  • Master cycler (PCR) is an exponentially progressing synthesis of the defined target DNA sequences under in vitro.
  • PCR used for classification of organisms, genotyping, molecular archaeology, mutagenesis, mutation detection, sequencing, cancer research, detection of pathogens, DNA fingerprinting, drug discovery, genetic matching and engineering.

Multiporator, Eppendorf India Limited

  • Utilzeed for the electroporation of eukaryotic cells, bacteria and other microorganisms, as well as the electrofusion of cells possible with Eppendorf  Multiporator modular system. The applications includes
  • Makes stable and transient transfection of eukaryotic cells 
  • Efficient and gentle transfer of siRNA
  • Electroporation of bacteria, yeasts and other microorganisms
  • Generation of hybridoma cells for the production of monoclonal antibodies
  • Fusion of plant protoplasts 

Real-time PCR , Eppendorf India Limited

  • Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets.
  • This system has powerful assay design algorithms, optimized master mixes, intuitive data analysis software and flexible instrumentation to harness the power of qPCR across a rich and diverse set of applications. The applications include
  • Gene Expression (mRNA) analysis
  • microRNA & Noncoding RNA analysis
  • Genetic variation analysis
  • Mutation detection Assays

Probe Sonicator

  • Sonication is the act of applying sound energy to agitate particles in a sample, for various purposes. Ultrasonic frequencies (>20 kHz) are usually used, leading to the process known asr ultra-sonication.
  • Sonicators are used for a wide variety of applications including: Protein purification, Degassing, PMCA, Sono-chemistry, Liposomes, Crystallization, Emulsification, Extraction,  Transesterification, focused Cleaning,  and solubilize the compounds

Binary Gradient HPLC system, Shimadzu

  • This instrument is used to separate the components in a mixture to identify each component, and to quantify each component.
  • It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material.
  • Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the effective separation of the components as they flow out of the column.

Thermostat C, Eppendorf India Limited

  • The ideal device to accurately set and maintain temperatures. The ThermoStat C features excellent temperature management.
  • Provides comfortable and precise temperature control of all conventional lab vessels. 
  • The operation is easier and more intuitive than ever before.
  • Predefined temperature keys provide quick access to five important experimental temperatures.
  • Due to comprehensive program functions, one can also individually store 15 programs. With an extensive range of exchangeable Smart Blocks, the ThermoStat C adapts to any application.

Gel Doc, Labmate (Asia) PVT LTD

  • A gel doc, also known as a gel documentation system, gel image system or gel imager, is an equipment widely used in molecular biology laboratory for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels.
  • Generally, a gel doc includes an ultraviolet (UV) light transilluminator, or  darkroom to shield external light sources and protect the user from UV  exposure, and a 
  • CCD camera for image capturing

Table-top Centrifuge, Eppendorf 

  • A mini spin (centrifuge) is a piece of equipment, generally driven by an electric motor
  • The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances to separate out along the radial direction. 
  • Simple centrifuges are used in chemistry, biology and biochemistry for isolating and separating suspensions.

Gel Electrophoresis Vertical and Horizontal, BioRad

  • Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
  • Estimation of the size of DNA molecules following restriction enzyme digestion, e.g. in restriction mapping of cloned DNA.
  • Analysis of PCR products, e.g. in molecular genetic diagnosis or genetic fingerprinting
  • Separation of restricted genomic DNA prior to Southern transfer, or of RNA prior to Northern transfer.
  • Gel electrophoresis is used in forensics, molecular biology, genetics, microbiology and biochemistry.

 


Multimode Reader with Alpha Tech, PerkinElmer

  • The benchtop multimode instrument to feature label-free technology 
  • Ultra-sensitive luminescence option for detecting fewer cells than standard luminescence, ideal for primary, stem or difficult-to-transfect cells 
  • Quad-monochromator for fluorescence and absorbance – select any wavelength and perform wavelength scans for the best possible signal to background for fluorescence applications
  • Time-resolved fluorescence provides high sensitivity for biological and cellular assays, even when sample is at a premium or in low concentration.

 

 

Western Blot Semi Dry, Biorad 

  • Western electroblotting is the electrophoretic transfer of separated proteins in a gel to the surface of a thin support membrane matrix where they are bound and immobilized.
  • Semi-dry blotting is performed with plate electrodes in a horizontal configuration, sandwiching a gel and membrane between sheets of buffer-soaked filter paper that function as the ion reservoir. 
  • Western transfer amino acid analysis solid phase assay systems
  • Protein sequencing Western transfer, amino acid analysis solid phase assay systems.
  • Southern and northern transfers solid phase immobilization dry chemistry test strips, enzyme immobilization, gene probe assays

Phase Contrast Inverted Microscope, Micros Produktions

  • Phase contrast microscopy is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image.
  • The phase contrast microscope shows the contrast differences between cells.
  • This microscope has stable time-lapse imaging with automatic focus correction system
  • Improved performance in broader wavelength range Compatible with plastic dishes and well plates

Upright Freezer, Eppendorf and Deep Freezer, Thermo Fisher Scientific

  • Deep freezer, a stand-alone freezer unit for preserving research samples
  • This deep freezer generally can achieve lower than −80 °C 
  • Upright freezer from Eppendorf India Limited and other deep Freezer from Thermo Fisher Scientific are available in the school.

 

 

Autoclave, Technico

  • An autoclave is a pressure chamber used to sterilize equipment and media by subjecting them to high pressure saturated steam at 121 °C for around 15–20 minutes depending on the size of the load and the contents.
  • Sterilization autoclaves are widely used in microbiology, medicine, podiatry, tattooing, body piercing, veterinary science, mycology, dentistry, and prosthetics fabrication. They vary in size and function depending on the media to be sterilized.

Centrifuge 5810R, Eppendorf India Limited 

  • A centrifuge is a piece of equipment, generally driven by an electric motor and this centrifuge has refrigeration option to use temperature sensitive sample.
  • The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances to separate out along the radial direction. By the same token lighter objects will tend to move to the top.
  • Simple centrifuges are used in chemistry, biology and biochemistry for isolating and separating suspensions.

 


TOP